A-level Biology

a-level Biology

Overview of AQA A-level Biology qualifications

Subject content:

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1. Biological molecules

Syllabus componentContent
Monomers and polymers• The biochemical basis of life
• Monomers (e.g., monosaccharides, amino acids, nucleotides)
• Polymers
• Condensation Reaction
• Hydrolysis reaction
Carbohydrates• Monosaccharides: The building blocks of carbohydrates (e.g. glucose, galactose, fructose)
• Condensation Reaction
• Formation of Disaccharides: Maltose, sucrose, lactose
• Polysaccharides: Glycogen and starch, Cellulose
• Structure and functions of: glycogen, starch and cellulose in animal and plant cells
• Biochemical tests using Benedict’s solution to determine reducing and non-reducing sugars, and potassium iodide/potassium iodide to determine starch.
Lipids• Triglycerides: Formed from glycerol and three fatty acids by condensation to form ester bonds
• R-group of a fatty acid: saturated and unsaturated
• Phospholipids
• The different properties of triglycerides and phospholipids are due to their different structures.
• Emulsion test for the detection of lipids
Proteins• General properties of proteins:
– Amino acids, and the general structure of an amino acid
– Formation of Dipeptides and Polypeptides
– Role of hydrogen bonds, ionic bonds and disulfide bridges in protein structure
– Functions of proteins
– Relation between primary, secondary, tertiary and quaternary structure and function of protein
– Biuret test for the determination of proteins

• Proteins in the role of enzymes:
– A model of induced compliance of enzyme action
– Enzyme properties
Nucleic acids• Structure of DNA and RNA
• DNA replication
ATPDefinition and formation of ATP
WaterProperties of water
Inorganic ionsTypes, role and properties of ions

2. Cells

Syllabus componentContent
Structure of eukaryotic cells• cell surface membrane
• nucleus
• mitochondrion
• chloroplasts (in plants and algae)
• Golgi apparatus and Golgi vesicles
• lysosomes
• ribosomes
• rough endoplasmic reticulum and smooth endoplasmic reticulum
• cell wall (in plants, algae and fungi)
• cell vacuole (in plants).
Structure of prokaryotic
cells and of viruses
• Structure of prokaryotic cells and viruses
• Differences from eukaryotic cells
Methods of studying cells• Principles and limitations of microscopes
• Optical microscopes: measuring size, understanding magnification and differences in resolution.
• Use of the formula: Principles of cell fractionation and ultracentrifugation as used to separate cell components
The emergence
of cells from other cells
• Mitosis
• The stages of the cell cycle: interphase, prophase, metaphase, anaphase, and telophase (including cytokinesis)
• The appearance of cells at each stage of mitosis
Transport across
cell membranes
• simple diffusion
• facilitated diffusion
• osmosis
• active transport
• co-transport
Cell recognition
and the immune system
• Antigen definition
• Phagocytosis of pathogens
• Reaction of T-lymphocytes to foreign antigen (cellular response)
• The role of antigen-presenting cells in the cellular response
• The role of helper T cells (TH cells) in the stimulation of cytotoxic T cells (TC cells), B cells and phagocytes
• B-lymphocyte response to foreign antigen, clonal selection and release of monoclonal antibodies (humoral response)
• Definition of an antibody
• Structure of an antibody
• Formation of antigen-antibody complex
• The role of plasma cells and memory cells in the production of primary and secondary immune response
• The use of vaccines to protect individuals and populations from disease. The concept of herd immunity
• Differences between active and passive immunity
HIV• The structure of human immunodeficiency virus (HIV) and its replication in helper T cells
• How HIV causes the symptoms of AIDS. Why antibiotics are ineffective against viruses
• The use of monoclonal antibodies
• Ethical issues surrounding the use of vaccines and monoclonal antibodies
• The use of antibodies in the ELISA test

3. Organisms exchange substances with their environment

Syllabus componentContent
Surface area to volume ratio• Surface Area to Volume Ratio
• Adaptations in Larger Organisms
• Metabolic Rate
Gas exchange• Adaptations to gas exchange: unicellular organisms, insects, fish, plants.
• Structural and functional trade-offs between gas exchange efficiency and water loss in terrestrial insects and xerophytes.
• The human gas exchange system
• Ventilation
• Effects of disease and environmental pollution
Digestion and absorption• Digestion Overview
• Mammalian Digestion
• Absorption in the Ileum
Mass transport• Mass transport in animals:
– Hemoglobins and Hemoglobin
– The role of hemoglobin and red blood cells in oxygen transport
– Bohr effect
– General scheme of blood circulation in mammals
– General structure of the human heart
– The structure of arteries, arterioles, and veins as related to their function
– Structure of capillaries and formation of tissue fluid

• Mass transport in plants:
– Xylem
– Cohesion-tension theory of water transport in xylem
– Phloem
– Mass flux hypothesis for the mechanism of translocation in plants
– The use of tracer and ring experiments to study transport in plants

4. Genetic information, variation and relationships between organisms

Syllabus componentContent
DNA, genes and
chromosomes
• DNA in Cells: Prokaryotes, Eukaryotes
• Genes
• Eukaryotic DNA
DNA and protein synthesis• The genome and the proteome
• RNA structure: mRNA, tRNA
• Transcription as the production of mRNA from DNA
• The role of RNA polymerase in joining mRNA nucleotides
• Translation as the production of polypeptides from the sequence of codons carried by mRNA
• The roles of ribosomes, tRNA and ATP
Genetic diversity• Gene mutations
• Mutagenic agents
• Meiosis and the process of meiosis
Genetic diversity and adaptation• Natural selection and its principles in the evolution of populations
• Directional selection
Species and taxonomy• Species Identification
• Phylogenetic Classification
• Binomial Nomenclature
• Taxonomic Systems
Biodiversity within a community• Biodiversity
• Species Richness
• Index of Diversity
• Impact of Farming
Investigating diversity• Genetic diversity estimated by comparison:
– Observed characteristics
– DNA base sequences
– The base sequences of mRNA
– Amino acid sequences of proteins encoded by DNA and mRNA

• Quantitative investigations of variation within a species

5. Energy transfers in and between organisms

Syllabus componentContent
Photosynthesis• Light-dependent reaction
• Light-independent reaction (Calvin cycle)
• Environmental factors that limit photosynthesis.
Respiration • Glycolysis and its steps
• Anaerobic Respiration
• Aerobic Respiration
Energy and ecosystems• Plants and Biomass
• Gross Primary Production (GPP)
• Net Primary Production (NPP)
• Net Production of Consumers (NPC)
• Primary productivity
• Secondary productivity
Nutrient cycles• Nutrient recycling
• Role of saprobionts in decomposition
• Role of mycorrhiza in facilitating plant uptake of water and inorganic ions
• Role of bacteria in nitrogen cycling

6. Organisms respond to changes in their internal and external environments

Syllabus componentContent
Internal and external stimuli
and reactions
• Survival and response
• Receptors
• Control of heart rate
Nervous coordination• Nerve impulses
• Synaptic transmission
Skeletal muscle contraction by nerves• Muscle Contraction
• Muscle Structure
• Key Components in Contraction: Actin and Myosin, Calcium Ions and ATP, Calcium Ions and Tropomyosin, ATP and Phosphocreatine
• Muscle Fibre Types: Slow Muscle Fibres and Fast Muscle Fibres
Homeostasis• Principles of homeostasis and negative feedback
• Control of blood glucose concentration
• Control of blood water potential

7. Genetics, populations, evolution and ecosystems

Syllabus componentContent
Inheritance• Genotype and Phenotype
• Alleles
• Genetic Diagrams
• Statistical Analysis: Use of the chi-squared test to compare observed and expected phenotypic ratios
Populations• Species and Populations
• Genetic Concepts: Gene Pool, Allele Frequency
• Hardy–Weinberg Principle
Evolution and Speciation• Variation and Genetic Factors
• Natural Selection
• Speciation Types: allopatric (geographic separation) and sympatric (same area) speciation
• Genetic Drift
Populations in ecosystems• Community and Ecosystem
• Estimating Population Size
• Ecosystem Dynamics and Succession
• Involves managing succession to conserve habitats

8. The control of gene expression

Syllabus componentContent
Gene mutations in the process
of DNA replication
• Understanding mutations
• Consequences of mutations
Gene expression• Totipotent Cells
• Types of Stem Cells: Pluripotent, Multipotent and Unipotent, Unipotent, Induced Pluripotent Stem Cells (iPS)
• Stem Cell Applications
• Regulation of transcription and translation
• Gene expression and cancer
Using genome projects• Sequencing projects
• Applications
• Complex organisms
• Advances
Gene technologies• Recombinant DNA technology
• Use of labeled DNA probes and DNA hybridization to locate specific gene alleles
• Genetic fingerprinting

Assessment

Type of assessmentQuestionsFinal scoreWeighting of
final grade
Paper 1
76 points: mixture of short- and extended-response questions
15 points: extended response questions

Any content from topics 1-4, including relevant practical skills
91 marks35% of
A-level
Paper 276 points: mixture of short and extended answer questions
15 points: comprehension question

Any content from topics 5-8, including relevant practical skills
91 marks35% of
A-level
Paper 338 points: structured questions that include practical techniques
15 points: critical analysis of the experimental data presented
25 points: one essay with a choice of two titles

Any content from topics 1-8, including relevant practical skills
78 marks30% of
A-level

Required practical activities

1. Investigate how a specific variable affects the rate of an enzyme-controlled reaction.

2. Prepare stained squashes of plant root tip cells, and use an optical microscope to identify mitosis stages and calculate the mitotic index.

3. Create a dilution series of a solute to produce a calibration curve for determining the water potential of plant tissue.

4. Investigate how a specific variable impacts the permeability of cell membranes.

5. Dissect a gas exchange or mass transport system in plants or animals, or an organ within such a system.

6. Apply aseptic techniques to study how antimicrobial substances affect microbial growth.

7. Use chromatography to analyze pigments from leaves of different plants, such as shade-tolerant and shade-intolerant plants or leaves of various colors.

8. Investigate how a specific factor influences dehydrogenase activity in chloroplast extracts.

9. Investigate the effect of a specific variable on the respiration rate of single-celled organism cultures.

10. Examine how an environmental variable affects animal movement using a choice chamber or maze.

11. Create a dilution series of a glucose solution and use colorimetry to develop a calibration curve to determine glucose concentration in an unknown “urine” sample.

12. Investigate how an environmental factor influences the distribution of a particular species.

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Weighting of assessment objectives for A-level Biology

Exams will assess students on the following objectives:

AO1: Demonstrate knowledge and understanding of scientific concepts and methods.
AO2: Apply knowledge in theoretical and practical contexts, including data handling.
AO3: Analyze, interpret, and evaluate scientific information to make judgments, draw conclusions, and improve practical designs and methods.

Assessment objectives AOs*Paper 1
(%)
Paper 2
(%)
Paper 3
(%)
Total Weighting
(%)
AO144–4823–2728–3230–35
AO230–3452–5635–3940–45
AO320–2419–2331–3525–30
Total weight of components353530100
*Assessment Objectives (AOs) are set by Ofqual and are consistent across all A-level Biology specifications and exam boards.

Assessment weightings

Marks will be scaled to meet component weightings. Final marks are the sum of scaled component marks. Grade boundaries are set using total scaled marks.

СomponentMaximum raw markScaling factorMaximum scaled mark
Paper 191×191
Paper 291×191
Paper 378×178
Total scaled mark:260

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